Laboratory Techniques Training

We offer Guided online sessions designed for students to learn essential laboratory skills from the comfort of their homes. - Our online courses are structured to explain core experimental principles and protocols with practical video demonstrations and interactive modules.
Fields and Basic Experiments Covered:

• Biochemistry:

Spectrophotometry, protein estimation assays (e.g., Lowry, Bradford), buffer preparation, and basics of chromatography.

• Microbiology:

Aseptic techniques, preparation of culture media, Gram staining, simple staining, and introduction to antibiotic sensitivity testing.

• Molecular Biology:

DNA/RNA extraction (demonstration-based), PCR theory and setup, agarose gel electrophoresis, and primer design tutorials.

• Cell culture:

• Introduction to cell culture, types of cell lines (adherent vs. suspension), and essential laboratory equipment (biosafety cabinet, CO₂ incubator, microscope, etc.).

• Aseptic techniques, including sterile handling, use of laminar flow hoods, and contamination control.

• Preparation of cell culture media, supplementation with fetal bovine serum (FBS), antibiotics, and pH buffering systems.

• Thawing and revival of cryopreserved cells (demonstration-based), including the use of DMSO and proper handling post-thaw.

• Subculturing (passaging) of adherent cells, involving trypsinization, splitting ratios, and confluency monitoring.

• Cell viability assays, such as Trypan Blue exclusion, and growth monitoring through cell counting and microscopy.
  • Detection of contamination, including bacterial, fungal, and mycoplasma contamination by visual inspection and demonstration of staining/PCR-based methods.
• Cryopreservation basics, including freezing medium preparation, controlled-rate freezing, and long-term storage in liquid nitrogen.

• Introduction to drug treatment assays, including preparation of treatment concentrations, exposure of cultured cells, and viability testing using MTT or similar methods.

- Ideal for undergraduate and postgraduate students seeking to strengthen laboratory understanding in Biochemistry, Microbiology, Cell culture, and Molecular Biology.

- Course materials include video lectures, e-manuals, experiment simulations, and assessment-based certifications.

Certification

• A certificate of completion is awarded after successful participation and evaluation
• Support in writing mini-project reports or literature reviews based on the internship topic

This program is ideal for students preparing for higher studies, thesis projects, or those pursuing careers in research and diagnostics.